HeLa Cell Line

Origins of the HeLa Cell Line:

The HeLa cell line is an immortal cancer cell line. It is termed “immortal” because the cells can replicate indefinitely in culture. The original cells came from a visible malignant cervical tumor in the body of a 31-year-old African American woman named Henrietta Lacks. The cells were taken early in 1951 and given to Dr. George Gey, who produced the original cell line. Henrietta Lacks died the same year, in October, from the very cancer cells that have since been used to save thousands and thousands of lives.

Now there are many strains of HeLa cells all over the world that have evolved in cell culture, and it is estimated that more HeLa cells have been cultured than were in Henrietta Lacks body to begin with.

Contamination Problems with HeLa:

HeLa is a popular research tool because of the line’s remarkable durability and immortal nature. They are so durable, in fact, that in 1974, a researcher named Walter Nelson-Rees suggested that all cell cultures have been contaminated with HeLa. This turned out to be true, and the uncontrolled growth of HeLa is such a problem in many labs that, if the lab wants to use HeLa, it cannot use any other cell line in fear of compromising good science. It has been demonstrated that 10-20% of existent cell lines have been infiltrated by HeLa. Their generation time is a mere 24 hours, and, years later, they are still some of the strongest cells known to science. In fact, citing their ability to replicate, the disparity between human and HeLa cells, and their ability to proliferate unchecked despite human attempts to contain them, some scientists have described HeLa as its own species, and a highly successful one, at that.

Controversy Surrounding HeLa:

There is no requirement to inform a patient or their relatives about bodily material obtained during surgery, diagnosis, or therapy. The HeLa cell line sparked a 1950s debate on medical ethics because the HeLa cells were propagated, and later commercialized to a great extent, without the knowledge or permission of Lacks or her family. Infact, the HeLa cell line’s sheer ubiquity is how Henrietta Lacks’ family, far removed from the field of scientific research, came to know about it 24 years after the death of Henrietta Lacks.

Cytogenetics:

HeLa cells are anueploid, and have a modal chromosome number of 82, with four copies of chromosome 12 and three copies of chromosomes 6, 8, and 17. The modal number ranges from 70 to 164. 98% of cells display a small telocentric chromosome, and four typical HeLa marker chromosomes have been reported in the literature.

Links:

HeLa (ATCC)

siRNA Delivery – In Vivo Transfection Kits

CRO Pre-clinical Research Services: Xenograft animal models

GLP-compliant Cell Banking services

Generation of Stably Expressing Cell Lines in 28 Days

Stable RNAi Cell Line Generation: Stable Gene Knockdown

In Vivo siRNA Delivery: Tissue-targeted siRNA

Encapsulation of Protein, RNA, mRNA, and DNA Molecules into Liposomes

The Amazing Cells of Henrietta Lacks

Henrietta’s Dance

HeLa (Wikipedia)